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Rat Aortic Endothelial Cells are isolated from tissue of 1-day-old neonatal Sprague-Dawley rats. Rat Aortic Endothelial Cells are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture
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Image Search Results
Journal: Journal of Neurovirology
Article Title: Effects of cigarette smoke and HIV-1 factors on blood-brain barrier integrity and permeability in an in vitro model
doi: 10.1007/s13365-025-01295-2
Figure Lengend Snippet: Depiction of the sequence of experiments performed. ( a ) Phase contrast image of confluent Sprague-Dawley rat brain microvascular endothelial cells were seeded on ( b ) transwell inserts for measurement of Trans-Epithelial/Endothelial Electrical Resistance (TEER). The cells were also cultured on ( c ) chambered cell chambered cell culture slides, which were subsequently processed for immunofluorescence imaging following staining with specific primary antibodies against ZO-1 and claudin-5. See main text for details. Modified from Biorender
Article Snippet: Fig. 1 Depiction of the sequence of experiments performed. ( a ) Phase contrast image of confluent
Techniques: Sequencing, Cell Culture, Immunofluorescence, Imaging, Staining, Modification